easysep human naive cd4+ t-cell enrichment kit  (STEMCELL Technologies Inc)

Journal: mBio

Article Title: Ex vivo and in vivo HIV-1 latency reversal by “Mukungulu,” a protein kinase C-activating African medicinal plant extract

doi: 10.1128/mbio.03816-24

Figure Lengend Snippet: Baseline properties of primary cell samples obtained from 10 ART-suppressed PLWH. ( A ) Percent CD3+ CD4+ T-cells in PBMC. ( B ) Provirus levels in isolated CD4+ T-cells from each donor, as measured by IPDA. In panels A and B , shapes and colors denote samples obtained from study participants as annotated in . ( C ) Numerical summaries of data presented in panels A and B .

Article Snippet: CD4+ T-cells were isolated from frozen PBMCs from five study participants using the EasySep Human CD4+ T-cell Enrichment Kit (Stemcell Technologies).

Techniques: Isolation

Journal: mBio

Article Title: Ex vivo and in vivo HIV-1 latency reversal by “Mukungulu,” a protein kinase C-activating African medicinal plant extract

doi: 10.1128/mbio.03816-24

Figure Lengend Snippet: Magnitude of Mukungulu reactivation in PBMC correlates with intact provirus levels in CD4+ T-cells . ( A–F ) Correlations of viral protein levels in PBMC pellets following 72 hours treatment with anti-CD3/CD28 ( A–C ) or 1 µg/ml Mukungulu ( D–F ), as measured by Simoa, relative to total provirus ( A and D ), total defective provirus ( B and E ), and intact proviruses ( C and F ) in isolated CD4+ T-cells. In all panels, colors/shapes denote individual donors as annotated in . ( G ) Correlations of viral protein production relative to provirus levels in isolated CD4+ T cells obtained from 10 PLWH. Unfilled shapes denote gag-p24 protein below the LOD which is annotated here at 0.005 pg/mL. In panel G , light green shading denotes P < 0.05, and dark green shading denotes P < 0.005, as measured by ANOVA.

Article Snippet: CD4+ T-cells were isolated from frozen PBMCs from five study participants using the EasySep Human CD4+ T-cell Enrichment Kit (Stemcell Technologies).

Techniques: Isolation

Journal: mBio

Article Title: Ex vivo and in vivo HIV-1 latency reversal by “Mukungulu,” a protein kinase C-activating African medicinal plant extract

doi: 10.1128/mbio.03816-24

Figure Lengend Snippet: Mukungulu reactivates HIV expression in CD4+ T-cells obtained from five ART-suppressed PLWH. ( A ) Percent cell viability in the presence of LRAs after 72 hours of treatment. Results are presented relative to the viability of untreated cells cultured in parallel. ( B and C ) Detection of gag-p24 protein in cell pellets ( B ) and culture supernatants ( C ) after 72 hours of treatment with LRAs, as measured by Simoa. In all panels, blue values denote average fold increases over no-treatment controls. Shapes located on the dotted line denote gag-p24 protein below the LOD, which is annotated here at 0.005 pg/mL. *, P < 0.05 as measured by paired t -test.

Article Snippet: CD4+ T-cells were isolated from frozen PBMCs from five study participants using the EasySep Human CD4+ T-cell Enrichment Kit (Stemcell Technologies).

Techniques: Expressing, Cell Culture

Journal: mBio

Article Title: Ex vivo and in vivo HIV-1 latency reversal by “Mukungulu,” a protein kinase C-activating African medicinal plant extract

doi: 10.1128/mbio.03816-24

Figure Lengend Snippet: Mukungulu reverses HIV-1 latency in vivo. ( A ) Schematic illustration of the BLT humanized mice model-based study. ( B ) pVL rebound measurements in ART-suppressed BLT humanized mice, treated with Mukungulu. ( C ) Viral RNA induction from isolated human CD4+ T-cells from ART-suppressed mice treated with PBS vehicle control or Mukungulu. Colors/shapes denote individual mice. *, P < 0.05 as measured by the one-sided Mann-Whitney test.

Article Snippet: CD4+ T-cells were isolated from frozen PBMCs from five study participants using the EasySep Human CD4+ T-cell Enrichment Kit (Stemcell Technologies).

Techniques: In Vivo, Isolation, Control, MANN-WHITNEY